Chinese name: 5-Bromo-4-chloro-3-indolyl caprylate

English name: 5-Bromo-4-chloro-3-indoxyl caprylate

Abbreviation: X-Caprylate

CAS No.: 129541-42-0

Molecular Formula: C16H19BrClNO2

Molecular Weight: 372.69

Specification: 1 g / 5 g / 25 g (custom packaging supported)

Appearance: off-white crystalline powder

Catalog No.: G02020001

Color: Blue

Stock: In stock

X-Caprylate is also a C8 esterase chromogenic substrate. After enzymatic hydrolysis, it forms a blue precipitate, providing a chromogenic signal different from magenta for the culture medium. In chromogenic culture medium formulation development, this different color performance provides more choices for substrate selection, colony color design, and construction of multi-substrate systems.

The key difference is that X-Caprylate does not change the enzymatic detection logic; it only provides another color output. For formulations that have already established a C8 esterase detection system, replacing the substrate can obtain a different chromogenic signal without re-validating the enzymatic route.

Around the C8 esterase chromogenic substrate, the application of X-Caprylate can extend from culture medium formulation development to chromogenic system evaluation and enzyme activity screening.

1. Salmonella Chromogenic Culture Medium Formulation Development

Used for formulation development of Salmonella chromogenic culture media and selective differential culture media. By responding to C8 esterase-related activity, it forms a blue chromogenic signal, providing a color choice different from magenta substrates for culture medium R&D, and assisting in evaluating the differentiation effect between target bacteria and background flora.

2. Chromogenic Culture Medium Substrate Screening and Formulation Optimization

Used for substrate selection and formulation optimization of microbial chromogenic culture media. By comparing the chromogenic performance, background reaction, and compatibility with other chromogenic components of different substrates in specific culture systems, it provides raw material choices for chromogenic signal design and formulation adjustment.

3. Construction of Multi-Substrate Chromogenic Systems

Used for formulation design of multi-enzyme chromogenic systems in Salmonella chromogenic culture media. The blue signal can be combined with other chromogenic signals such as magenta and blue, assisting in constructing a multi-dimensional colony phenotype identification system and improving the differentiation between target bacteria and background flora.

✅ Stable physicochemical properties, easy for formulation integration

As a high-quality indole derivative, X-Caprylate has good solubility in conventional substrate solvents (such as DMF and DMSO), and exhibits excellent chemical stability during sterilization and refrigeration cycles, ensuring batch-to-batch consistency of the final culture medium product.

✅ Blue precipitate, clear colony boundaries

The blue precipitate produced after hydrolysis of X-Caprylate is insoluble in water and tightly deposits at the colony position without diffusing in the culture medium. Colony boundaries are distinct, facilitating counting and picking single colonies for subsequent confirmation. In multi-substrate systems, the non-diffusing precipitate also ensures that color signals do not interfere with each other.

✅ Mature patented route, formulation design has a basis

As a blue substrate used to indicate C8 esterase activity in Salmonella chromogenic culture media, X-Caprylate has clear application paths for reference in multiple patents/technical solutions related to chromogenic culture media.

✅ Flexible specification options, convenient for R&D validation

For R&D and procurement needs at different stages, GeneSeqTools Bioscience & Technology provides X-Caprylate in stock, with specifications covering 1 g, 5 g, and 25 g, and supports customized packaging, making it convenient for customers to arrange procurement based on experimental validation and subsequent usage.

Magenta-Caprylate and X-Caprylate are both C8 esterase chromogenic substrates and can be used for formulation development of related microbial chromogenic culture media. The two have different chromogenic products and can be compared and evaluated in combination with the target formulation.

Which one to choose can be determined based on the following factors:

• Color and transparency of the culture medium base: The base color, transparency, and indicator components of the culture medium may affect the final chromogenic performance. The discernibility of blue and magenta signals should be compared in combination with the target formulation.

• Background interference caused by sample type: For complex background flora in food, feed, and environmental samples, the actual colony colors of target and non-target bacteria should be combined to evaluate the interpretation performance of different chromogenic substrates.

• Interpretation habits of end users: Different laboratories have different sensitivities and preferences for color. Providing two choices can cover a wider range of customer needs.

• Product line differentiation: Different products from the same company use different color identifications, making it convenient for operators to quickly identify.

GeneSeqTools Bioscience & Technology supplies both C8 esterase substrates simultaneously, allowing customers to obtain two color options from the same supplier and flexibly evaluate formulation compatibility.