Chinese Name: β-Alanine 7-amido-4-methylcoumarin trifluoroacetate

English Name: H-β-Ala-AMC ∙ TFA

Abbreviation: β-Alanine 7-amido-4-methylcoumarin trifluoroacetate

CAS Number: 201847-54-3

Molecular Formula: C15H15F3N2O5

Molecular Weight: 360.29

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Product Number: G04050017

Stock: In stock

Luminescence: Blue fluorescence

The working mechanism of H-β-Ala-AMC ∙ TFA follows a classic "structure-event-signal" logic chain. In the unreacted state, the AMC fluorophore is tightly linked to β-alanine via an amide bond, locking the molecule's fluorescence properties. When a sample containing the specific aminopeptidase is introduced into the system, the enzyme precisely recognizes the β-alanine residue and acts like a pair of molecular scissors, cleaving the amide bond. This cleavage event instantly releases free AMC molecules, which then emit a strong blue fluorescence signal under UV excitation. The signal intensity is proportional to the enzyme activity and can be read continuously in real-time using a fluorescence microplate reader.

• Quantitative Detection of Aminopeptidase Activity

Utilizing its high fluorescence quantum yield for micro-kinetic analysis of samples containing specific aminopeptidases (such as those targeting β-alanine sequences) in in vitro biochemical experiments.

• Inhibitor Screening and Drug Development

Suitable for high-throughput screening systems to rapidly evaluate the inhibitory effects of candidate molecules on aminopeptidases.

• Rapid Microbial Phenotypic Identification

Because some pathogenic bacteria or specific cell lines possess unique enzymatic characteristics for degrading β-alanine substrates, this product can be integrated into culture media as a core chromogenic/luminescent raw material.

✅ Sensitive Signal

Strong fluorescence response upon AMC release, enabling detection of low-abundance enzyme activity and lowering the detection limit.

✅ Real-Time Readout

Fluorescence signal changes continuously with the reaction ; obtain kinetic curves directly without stopping the reaction.

✅ Low Background

Extremely weak fluorescence in the unhydrolyzed state , significantly improves signal-to-noise ratio for clearer, more reliable data.

✅ Three In-Stock Sizes

One-stop solution from development to scale-up: 1 g suitable for methodology exploration, 5 g for medium-scale validation, 25 g covering large projects or pilot-scale needs. Three specifications available from stock, with custom packaging also open — ensuring no delays caused by supply lead times.