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Recommended Series | Break Free from Import Dependence! Domestically Produced Chromogenic Substrate X-β-Xyl Now Available in Stock for Efficient β-D-Xylosidase Detection

2025/8/14 16:14:58 Browse volume(39)


β-D-Xylosidase Substrate

X-β-Xyl (CAS: 207606-55-1)


In microbial identification and enzyme activity assays, selecting an appropriate chromogenic substrate directly affects experimental efficiency and result accuracy.

How can you quickly identify target microorganisms in complex communities? How can you simplify β-D-xylosidase detection workflows? How can you get rid of the long lead times and high prices of imported reagents?

5-Bromo-4-chloro-3-indolyl β-D-xylopyranoside (X-β-Xyl) — the preferred domestic alternative, in stock, offering an ideal solution.

I. Working Principle

X-β-Xyl is a chromogenic substrate specifically designed for β-D-xylosidase. In the presence of the target enzyme, it specifically cleaves the β-glycosidic bond of the substrate, releasing colorless 5-bromo-4-chloro-3-indoxyl. This compound rapidly dimerizes under oxygen to form a striking blue precipitate—5,5'-dibromo-4,4'-dichloro-indigo. This “colorless-to-blue” chromogenic reaction makes enzyme activity detection visually apparent.

II. Application Scenarios

1. Microbial identification: Used in media to rapidly identify β-D-xylosidase-producing strains. Target genera such as Klebsiella and Enterobacter form characteristic blue colonies, in contrast to organisms that do not produce this enzyme (e.g., Escherichia coli), improving screening efficiency.

2. Enzyme activity quantification: In biochemical studies, this substrate can be used to detect β-D-xylosidase activity in purified enzymes or crude extracts, providing a reliable tool for enzymology research.

3. Gene expression analysis: The β-D-xylosidase gene can serve as a reporter gene; gene expression regulation can be studied via the X-β-Xyloside chromogenic reaction.


III. Product Overview

207606-55-1 structural formula

Chinese name: 5-Bromo-4-chloro-3-indolyl β-D-xylopyranoside

English name: 5-Bromo-4-chloro-3-indolyl β-D-xylopyranoside

Abbreviation: X-β-Xyl

CAS No.: 207606-55-1

Molecular formula: C13H13BrClNO5

Molecular weight: 378.60

Specification: 100 mg / 500 mg / 1 g (custom packaging supported)

Appearance: off-white lyophilized powder

Catalog No.: G01010018


IV. Core Advantages

1. High specificity: Designed specifically for β-D-xylosidase with high selectivity, significantly reducing interference from non-target enzymes and effectively lowering false positives.

2. High sensitivity: Under low enzyme activity, the blue precipitate gradually accumulates to yield clear coloration, ensuring low-abundance samples are not missed.

3. Intuitive results: The reaction product is an insoluble blue precipitate with vivid color and good stability, easily observed by eye or microscope without complex instruments.

4. Easy to use: Can be directly added to various solid or liquid media without special treatment; simple to operate and widely applicable to high-throughput screening and large-scale microbial identification workflows.


V. Related Product Recommendations

PNP-β-D-Xyl

2001-96-9 structural formula

Chinese name: 4-Nitrophenyl-β-D-xylopyranoside

English name: 4-Nitrophenyl-β-D-xylopyranoside

Abbreviation: PNP-β-D-Xyl

CAS No.: 2001-96-9

Molecular formula: C11H13NO7

Molecular weight: 271.23

Specification: 0.5 g / 1 g / 5 g (custom packaging supported)

Appearance: off-white lyophilized powder

Color: yellow



Extended Services

Every step of scientific research deserves to be precisely “seen”.

Choose the domestic β-D-xylosidase chromogenic substrate from Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd. to make every enzyme activity assay clearer, more efficient, and more reliable.

For related glycosidase substrates or custom services, please call +86 13302967066!

In vitro molecular diagnostics | Gene sequencing | Microbial testing


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Founded in 2015, it is a high-tech enterprise focusing on microbial testing、 gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.

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