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Recommended Series | X-Thymidine-3'-phosphate CHA Salt: Ensuring Standardized Testing from Microbial Identification to IHC/WB

2025/8/22 14:58:11 Browse volume(21)


Chromogenic Substrate for DNase

X-Thymidine-3'-phosphate CHA Salt


Can domestic reagents also achieve high stability? Yes.

Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd. uses cyclohexylammonium salt (CHA) to optimize water solubility and a lyophilization process to ensure batch-to-batch consistency,

making X-Thymidine-3'-phosphate CHA Salt (CAS: 341973-00-0) not only reliable in performance but also continuously available.

Standardized testing, no longer constrained by import shortages and price fluctuations.

I. Principle of Operation

X-Thymidine-3'-phosphate CHA Salt is a specific chromogenic substrate designed for detecting the activity of bacterial Deoxyribonuclease (DNase) and Phosphodiesterase II. Its detection mechanism is based on an enzymatic hydrolysis reaction: the target enzyme specifically cleaves the phosphodiester bond in the substrate molecule, releasing a 5-bromo-4-chloro-indolyl group. This product then spontaneously oxidizes and dimerizes in the air, forming an insoluble indigo-like dye. A distinct blue to blue-green precipitate appears at the reaction site, directly indicating the presence and location of enzyme activity, enabling rapid, visual interpretation.

II. Application Scenarios

1. Identification and differentiation of DNase-positive bacteria: Used to prepare DNase chromogenic agar, facilitating the rapid identification of DNase-producing bacteria through color reaction. Typical positive bacteria include:

  • Staphylococcus aureus

  • Pseudomonas aeruginosa

  • Some serotypes of Salmonella spp.

  • Streptococcus pyogenes

2. Immunodetection techniques: Compatible with chromogenic systems for protein detection in Immunohistochemistry (IHC) and Western Blot, facilitating methodological consistency and result alignment.

3. Environmental and food microbiology testing: Used for rapid screening of pathogenic bacteria with DNA degradation capabilities in food, clinical, or environmental samples, enhancing detection efficiency and accuracy.


III. Product Overview

Structure of 341973-00-0

Product Name: 5-Bromo-4-chloro-3-indolyl-D-thymidine-3'-phosphate cyclohexylammonium salt

English Name: 5-Bromo-4-chloro-3-indolyl-D-thymidine-3'-phosphate cyclohexylammonium salt

Abbreviation: X-Thymidine-3'-phosphate CHA Salt

CAS No.: 341973-00-0

Molecular Formula: C18H17BrClN3O8P · C6H14N

Molecular Weight: 649.86

Specification: 100 mg / 500 mg / 1 g (Custom packaging available)

Appearance: Off-white lyophilized powder

Cat. No.: G07010001


IV. Core Advantages

1. High Specificity: Specifically designed for bacterial DNase and Phosphodiesterase II, with minimal cross-reactivity with non-target enzymes, reducing the risk of false positives and ensuring reliable results.

2. Wide Application: Compatible with various detection systems, including microbial culture identification, IHC, and Western Blot. It is versatile and flexibly adapts to research and quality control workflows.

3. Good Stability: The cyclohexylammonium salt (CHA) form enhances water solubility and storage stability. The lyophilized powder is easy to store and transport, with high batch-to-batch consistency.

4. In Stock: Standard specifications are available from stock, shortening lead times and ensuring the continuous progress of research and testing processes.



Extended Services

Every step of scientific research deserves to be precisely "seen".

Choose X-Thymidine-3'-phosphate CHA Salt (CAS: 341973-00-0), to make every enzyme activity test clearer, more efficient, and more reliable.

For related nuclease substrates or custom services, please call +86 13302967066!

For related products such as hematology stains and buffer salts, you can view the complete product line and technical specifications on the [MedSun official website].

In Vitro Diagnostics | Gene Sequencing | Microbiology Testing


END


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Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.

Established in 2015, it is a high-tech enterprise focusing on the R&D, production, and sales of biochemical reagent raw materials for fields such as microbiology testing, gene sequencing, and in vitro molecular diagnostics. We are committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, with a product portfolio covering more than ten categories and nearly a thousand innovative products. Adhering to the core philosophy of "Customer First, Integrity and Professionalism," we serve with dedication to meet the diverse needs of customers in research and testing.

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