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Recommended Series | U-3ʹ-BCIP: Clearer Signals, Easier Detection—Specifically Designed for RNase and Phosphatase Assays
2025/8/29 15:20:03 Browse volume(11)U-3ʹ-BCIP
In an era of molecular diagnostics pursuing ultimate sensitivity, background noise is a hidden cost.
Amplifying weak RNase A events into clear, precipitating signals is becoming a common language from R&D to mass production.
The real solution starts at the source—choosing a chromogenic substrate that can thoroughly reduce background and significantly improve the signal-to-noise ratio.
I. Working Principle
U-3ʹ-BCIP is a high-sensitivity chromogenic substrate specifically designed for detecting the activity of Ribonuclease A (RNase A). Its mechanism is based on enzyme-specific hydrolysis: under the specific catalysis of RNase A, the phosphodiester bond in the substrate molecule is hydrolyzed, releasing an indole group. This group undergoes oxidative dimerization to form an insoluble blue/purple precipitate, accurately localizing the enzyme's active site.
The value of this mechanism, based on enzymatic reaction and colorimetric output, extends far beyond theory:
High Signal-to-Noise Ratio: The precipitating signal does not easily diffuse, resulting in extremely low background.
Strong Stability: The colored result is long-lasting, facilitating recording and analysis.
Broad Compatibility: Suitable for both solid-phase and liquid-phase detection systems.
II. Application Scenarios
1. Food Safety Monitoring: Acts as a high-sensitivity "hygiene indicator" to assess food cleanliness, contamination risk, and quality stability, providing early warnings for foodborne risks.
2. Clinical Adjuvant Diagnosis: Detects RNase A activity in body fluids, providing a reference for the diagnosis and prognostic assessment of diseases such as pancreatic function and malignant tumors.
3. RNase A Activity Assay: Used for quality assessment of recombinant RNase A and purity testing of enzyme preparations, featuring high sensitivity and rapid color development.
III. Product Overview
Chinese Name: Uridine-3'-(5-bromo-4-chloro-3-indolyl)-phosphate tetrabutylammonium salt
English Name: 5-Bromo-4-chloro-3-indolyl-uridine-3ʹ-phosphate tetrabutylammonium salt
Abbreviation: U-3ʹ-BCIP
CAS No.: 132900-87-9
Molecular Formula: C17H15BrClN3O9P · C16H36N
Molecular Weight: 794.12
Specification: 100 mg / 500 mg / 1 g (Custom packaging available)
Appearance: Off-white lyophilized powder
Catalog No.: G07020001
IV. Core Advantages
High Specificity: Structure optimized for RNase A recognition, reducing non-specific hydrolysis and eliminating "false positive" interference.
High Contrast: Lyophilization process plus molecular modification significantly reduces the rate of spontaneous hydrolysis, resulting in clean color development and easier result interpretation.
Precise Localization: Generates an insoluble precipitate for accurate signal localization, particularly suitable for in-situ detection and colony screening.
Reliable Supply: In-house domestic production system breaks import restrictions, supports flexible customization, and meets the needs of the entire chain from R&D to mass production.
Extended Services
Accurate enzyme activity detection originates from a reliable substrate choice.
With its excellent signal-to-noise ratio and stability, U-3ʹ-BCIP helps you achieve clearer and more credible experimental results.
For related nuclease substrates or custom services, please call +86-13302967066!
For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on the [MedSun official website].
Established in 2015, we are a high-tech enterprise specializing in the R&D, production, and sales of biochemical reagent raw materials for fields such as microbial detection, gene sequencing, and in vitro molecular diagnostics. We are committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, with a product portfolio covering more than ten categories and nearly a thousand innovative products. Adhering to the core philosophy of "Customer First, Integrity and Professionalism," we serve with dedication to meet the diverse needs of customers in research and testing.
GeneSeqTools Official Account: Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.
GeneSeqTools Website: http://www.geneseqtools.com
Hotline: +86-0755-23251735
QQ: 2370561481
Email: sales@geneseqtools.com
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