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Recommended Series | Acridinium Ester NSP-SA-NHS: The "Flashlight-Grade" Labeling Powerhouse for Chemiluminescence
2025/9/1 16:05:06 Browse volume(11)In the precise experiments of biomolecular labeling, signal detection is like searching for fireflies in the dark—faint, fleeting, and easily obscured by interference. Acridinium ester NSP-SA-NHS, however, acts like a precision molecular flashlight: requiring no trigger switch (enzyme catalysis), it emits an intense burst of photons within 2 seconds upon the "touch" of alkaline hydrogen peroxide, making the traces of antibodies and nucleic acids impossible to hide.
I. Product Properties
Chinese Name: Acridinium Ester (NSP-SA-NHS)
English Name: NSP-SA-NHS
CAS No.: 199293-83-9
Molecular Formula: C32H31N3O10S2
Molecular Weight: 681.74
Specification: 1mg/ 5mg/ 10mg (Custom packaging supported)
Form: Light yellow powder
Item No.: G05010001
Acridinium Ester (NSP-SA-NHS) is a compound specifically designed for efficient chemiluminescent labeling, offering excellent stability and sensitivity. Its powder form facilitates transportation and storage, making it suitable for various applications such as scientific research experiments and the production of in vitro diagnostic kits.
II. Application Scenarios
Chemiluminescence and Immunoassays: Widely used in tumor marker and infectious disease antibody/antigen diagnostic systems.
Receptor Assays: Precisely label biological molecules such as proteins, peptides, and antibodies for receptor binding studies.
Nucleic Acid and Peptide Detection: Suitable for nucleic acid and peptide detection, emitting light rapidly under excitation conditions, supporting high-throughput and real-time detection applications.
III. Product Advantages
Ultra-Fast Luminescence: Excited by hydrogen peroxide under alkaline conditions, releases photons within 2 seconds, compatible with high-speed detection equipment, improving experimental throughput.
Simple Operation: No enzyme participation required, simplifying reaction steps and reducing experimental complexity and time costs.
Broad Compatibility: Suitable for labeling various biological molecules such as proteins, peptides, antibodies, and nucleic acids, meeting diverse research needs.
Long-Lasting Activity: Its stable amide bond conjugation ensures the labeled compound maintains activity during long-term storage, reducing background interference.
IV. Detection Principle
Acridinium Ester (NSP-SA-NHS) specifically binds to amino-containing proteins: In an alkaline environment, the NHS group dissociates, allowing the acridinium ester to form a stable amide bond complex with the target molecule. After the reaction, unbound free acridinium ester can be rapidly removed via a desalting column, ensuring high purity of the labeling system. Upon addition of hydrogen peroxide in alkaline conditions, the acridinium-labeled conjugate produces instantaneous luminescence within 2 seconds, emitting photons at a wavelength of 430 nm. Due to the transient nature of this light signal, precise capture of the millisecond-scale light pulse requires the coordinated operation of a high-sensitivity luminometer and photon detector, enabling accurate quantitative analysis of the labeled compound concentration.
This technology can be widely applied to the labeling and detection of biological molecules such as proteins, antibodies, and nucleic acids, providing core support for fast, low-noise chemiluminescence systems.
Extended Services
We simultaneously provide a variety of chemiluminescent substrates and related raw materials. For more customized solutions or technical consultations, please call +86 13302967066!
For related products such as blood cell dyes and buffer salts, you can check the complete product line and technical specifications on the [MedSun Official Website].
Established in 2015, it is a high-tech company focusing on the research, development, production, and sales of biochemical reagent raw materials in fields such as microbial detection, gene sequencing, and in vitro molecular diagnostics. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product range covers more than ten categories and nearly a thousand innovative products. Upholding the core philosophy of "customer first, integrity and professionalism," we serve with dedication to meet the diverse needs of customers in scientific research and testing.
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