Chinese Name: Anaerobic Bacteria β-D-Glucosidase Substrate

English Name: Anaerobic β-D-glucosidase substrate

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Purity: HPLC ≥98%

Product Number: GST2020

Stock: In stock

Color: Orange (insoluble precipitate)

The Anaerobic Bacteria β-D-Glucosidase Substrate employs an intramolecular condensation chromogenic system, triggering structural transformation through enzymatic reaction to achieve stable color development under anaerobic conditions.

1. Enzyme recognition and bond cleavage: β-D-Glucosidase specifically recognizes and hydrolyzes the β-D-glucosidic bond in the substrate, releasing a colorless chromogenic precursor molecule.

2. Intramolecular condensation: The released precursor molecule undergoes spontaneous intramolecular condensation under anaerobic conditions without the need for oxygen or any auxiliary oxidant, forming a chromogenic intermediate with a conjugated structure.

3. Precipitation and color development: The condensation product is a stable orange insoluble dye that deposits in situ on the colony and fixes the signal, achieving clear and visible color development results.

1. Anaerobic bacteria chromogenic medium development

Used for screening β-D-glucosidase-positive bacteria and constructing differentiation media, achieving colony color distinction through enzyme activity differences.

2. Enzyme activity detection of β-D-glucosidase-positive strains

Used for screening and enzyme activity analysis of relevant enzyme-producing strains, enabling rapid differentiation between target and non-target bacteria.

✅ High purity control, ensuring detection consistency

• High purity quality control: HPLC ≥98% high-purity system ensures batch stability.

• Controllable background: Reduces non-specific chromogenic interference and improves interpretation stability.

• Batch stability: Strict quality control standards guarantee consistent color development performance across different batches.

✅ Stable color development under anaerobic conditions, adapted to oxygen-free detection systems

• Anaerobic color development: Color development is completed without the need for oxygen.

• Anaerobic/aerobic compatibility: Stable color development in both anaerobic and conventional culture systems.

✅ Orange signal, high‑visibility color performance

• Orange precipitate signal: Forms orange insoluble precipitate in situ after cleavage, with signal concentrated on the colony and no diffusion.

• Clear contrast: Orange color contrasts distinctly with most media backgrounds, facilitating rapid identification of positive colonies.

• Intuitive interpretation: Reduces the risk of missing weakly positive samples and improves colony screening efficiency.

✅ No toxic intermediate accumulation, ensuring detection sensitivity

• No auxiliary reagents required: No need for oxidants, coupled enzymes, or diazonium salts, simplifying the experimental process.

• Non‑toxic intermediates: The color development process does not generate toxic intermediates, does not inhibit strain growth, and ensures detection sensitivity.

• Fewer experimental variables: Reduces operational errors and batch fluctuations introduced by auxiliary reagents, providing stable and reliable results.

✅ In‑stock supply, cost‑effective alternative

• Price advantage: Leveraging the domestic supply chain, the cost structure is superior compared to imported raw materials.

• In‑stock supply: Sufficient inventory on hand, shortening procurement cycles and ensuring project progress.

• Flexible specifications: Available in 1 g / 5 g / 25 g, supporting needs from R&D to large‑scale production.